2009 PRRS Symposium Program
Updated Program - November 2, 2009
Organizing Committee:
Chair: Joan Lunney, USDA-ARS BARC
Co-Chair: Lisa Becton, National Pork Board
Scientific Program Chair: X.J. Meng, Virginia Polytechnic Institute and State Univ
Proceedings Editor: Raymond R.R. Rowland, Kansas State Univ
Scientific Program Committee:
Luis Enjuanes, Centro Nacional de Biotecnología (CSIC), Madrid, Spain.
Derald J. Holtkamp, Iowa State University
Michael Murtaugh, University of Minnesota
Eric A. Nelson, South Dakota State University
Asit K. Pattnaik, University of Nebraska-Lincoln
Montserrat Torremorell, University of Minnesota
Hanchun Yang, China Agricultural University, Beijing, China
Federico A. Zuckermann, University of Illinois
Friday, December 4th
14:00-17:30 PRRS Symposium Registration (7th floor lobby registration desk)
Pre-Symposium Meetings
Grand Ballroom, Salon III (7th floor)
13:30-14:30 NC-229 Business meeting, Joan Lunney, Chair
13:30 Introduction - Joan Lunney, Chair
13:35 Nominations of new officers and voting by station reps
13:40 Discussion of progress and plans by Objective, led by the respective Objective Coordinators
14:15 Reports from each Objective team
14:25 Closing
Friday, December 4th
USDA NIFA PRRS CAP Workshop
Grand Ballroom, Salon III (7th floor)
Europe, United States and China Perspectives on PRRS Research
Sponsored by the USDA NIFA PRRS CAP
Raymond (Bob) Rowland, Director
(browland@vet.k-state.edu)
14:30 Welcome - Bob Rowland
14:30 PoRRSCon, a European consortium that aims to develop new generation marker
vaccines and differential ELISAs in order to be able to control PRRS under
European circumstances.
Hans Nauwynck-Coordinator, Laboratory of Virology, Faculty of Veterinary Medicine,
Ghent University, Salisburylaan, Merelbeke, Belgium.
14:45 National Pork Board PRRS Virus Control.
Lisa Becton-Director of Swine Health Research and Information, National Pork Board, Des Moines, Iowa.
15:00 Studies on highly pathogenic PRRSV in China.
Shishan Yuan-Department of Swine Infectious Diseases, Shanghai Veterinary Research
Institute, The Chinese Academy of Agricultural Sciences, Shanghai, China.
15:15 PRRS Research, the perspective from China.
Hanchun Yang-College of Veterinary Medicine, China Agricultural University, Beijing,
China.
15:30 PRRS Coordinated Agricultural Project (CAP): Control and elimination of PRRSV.
Raymond (Bob) Rowland-Director, Department of Diagnostic Medicine/Pathobiology,
College of Veterinary Medicine, Kansas State University, Manhattan, Kansas.
15:45 Panel
2009 International PRRS Symposium
Molecular, Immunological, Genetic and Epidemiological Approaches for PRRSV Control
The 2009 International PRRS Symposium Program has been approved for 12 hours of continuing education credit in jurisdictions which recognize AAVSB RACE approval for veterinarians and veterinary technicians. Confirmation forms will be available at the PRRS Symposium Registration Desk; signed forms must be submitted at the registration desk before the end of the Symposium.
Keynote presentations: 60/50 minute total with a 50/40 minute oral presentation and a 10 minute interactive discussion.
Oral presentations: 20 minutes total with a 15 minute oral presentation and 5 minutes interactive discussion
Friday, December 4th
Opening Session
Co-Chairs: Joan Lunney and Lisa Becton
Grand Ballroom, Salon III (7th floor)
16:00 Welcome, Joan Lunney, Chair – 2009 Intl PRRS Symposium
16:10 Keynote Presentation #1:
Swine Influenza and the Need for Rationally Designed Vaccines.
Jürgen A. Richt, Kansas State University, Manhattan, KS
17:15-19:00 Reception and cash bar
17:15-19:00 Poster Session 1 (odd-numbered posters; Grand Ballroom, Salon III, 7th floor)
Saturday, December 5th
Session I: Virus-Host Interaction and Pathogenesis
Co-Chairs: Michael Murtaugh and Hanchun Yang
8:00 An infection model for the study of PRRS at the population level.
Bob Rowland. Kansas State University, Manhattan, KS
8:20 PRRSV NSP 1ß inhibits host type I IFN response by antagonizing IRF3 activation.
Lalit Beura. University of Nebraska, Lincoln, NE
8:40 Porcine reproductive and respiratory syndrome virus (PRRSV) nonstructural protein 1 (nsp1) functions as an interferon antagonist.
Ying Fang. South Dakota State University, Brookings, SD
9:00 Inhibition of type I interferon signaling by Nsp11 of PRRSV.
Dongwan Yoo. University of Illinois at Urbana-Champaign, Urbana, IL
9:20-10:40 Coffee break
9:20-10:40 Poster session 2 (even-numbered posters; Grand Ballroom, Salon III, 7th floor)
Session II: Molecular Biology: Structural and Functional Relationship
Co-Chairs: Luis Enjuanes and Asit Pattnaik
10:40 The role of N-glycosylation of minor envelope glycoproteins of PRRSV on infectious virus production and interaction with the receptor CD163.
Phani Das. University of Nebraska-Lincoln, NE
11:00 Viral N-acetylglucosamine Facilitates Porcine Reproductive and Respiratory Syndrome Virus Infection of Permissive Cells.
J. Li. University of Minnesota, St. Paul, MN
11:20 The structural proteins of the Chinese highly pathogenic porcine reproductive and respiratory syndrome virus do not contribute to its virulence.
Hanchun Yang. China Agricultural University, Beijing, China
11:40 Cis-Acting Elements in the 3' terminus of the genome of Porcine Reproductive and Respiratory Syndrome Virus are required for viral RNA synthesis.
Shishan Yuan. Chinese Academy of Agricultural Sciences, Shanghai, China
12:00 Lunch buffet
Session III: Epidemiology, Heterogeneity and Evolution
Co-Chairs: Montserrat Torremorell, and Derald Holtkamp
13:00 Keynote Presentation #2:
Genetic diversity of PRRSV - global emergence and evolution.
Tomasz Stadejek, National Veterinary Research Institute, OIE Reference Laboratory for PRRS, Poland
13:50 A Phylogeny-based Demographical and Geographical Dissection of North American Type Porcine Reproductive and Respiratory Syndrome Viruses.
Mang Shi. The University of Hong Kong, Hong Kong, China
14:10 Use of a production region model to evaluate aerosol transmission and biosecurity of PRRSV and Mycoplasma hyopneumoniae: Results from a 3-year study.
Scott Dee. University of Minnesota, St Paul, MN
14:30 Infectious dose (ID50) of PRRSV Isolate MN-184 for Young Pigs via Aerosol Exposure.
T.D. Cutler. Iowa State University, Ames, IA
14:50 PRRS Regional Eradication in Minnesota.
Cesar Corzo. University of Minnesota, St Paul, MN
15:10-15:40 Break (refreshments in the poster area)
Session IV: Vaccine and Immunity
Co-Chairs: Federico Zuckermann and Eric Nelson
15:40 Steps towards development of a novel mucosal vaccine to PRRSV.
Varun Dwivedi. Ohio State University, Wooster, OH
16:00 Proficient isolation and titration of field PRRS virus from clinical samples using a porcine alveolar macrophage cell line.
Federico Zuckermann. University of Illinois at Urbana-Champaign
16:20 Pathogenesis and antigenic characterization of a new East European subtype 3 porcine reproductive and respiratory syndrome virus strain.
Uladzimir U. Karniychuk. Ghent University, Merelbeke, Belgium
16:40 Modified live PRRS virus vaccination as a key component in nursery pig performance improvement.
G. Spronk. Pipestone Veterinary Clinic, Pipestone, MN
17:00 Adjourn—closing remarks, X.J. Meng, Scientific Program Chair
ABSTRACTS
2009 IPRRSS Keynote Speakers
Abstract No.
1- Swine Influenza and the Need for Rationally Designed Vaccines.
Jürgen A. Richt, DVM, PhD. Dept. Diagnostic Medicine and Pathobiology, Kansas State Univ., Manhattan, KS.
2- Origin, evolution and diversification of PRRSV.
Tomasz Stadejek1, Michael P. Murtaugh2. 1National Veterinary Research Institute, OIE Reference Laboratory for CSF and PRRS, Pulawy, Poland; 2Department of Veterinary and Biomedical Sciences, University of Minnesota, St. Paul, MN.
PRRS CAP Workshop Speakers
Abstract No.
3-PRRS Coordinated Agricultural Project (CAP): Control and elimination of PRRSV.
R.R.R. Rowland. Dept. Diagnostic Medicine and Pathobiology, Kansas State Univ., Manhattan, KS.
4-National Pork Board PRRS Virus Control.
L. Becton. Director of Swine Health Research and Information, National Pork Board, Des Moines, IA.
5- PoRRSCon, a European consortium that aims to develop new generation marker vaccines and
differential ELISAs in order to be able to control PRRS under European circumstances.
H. Nauwynck ,L. Enjuanes, E. Mateu, C. Vela, E. Giuffra, N. Ruggli, L. Larssen, T. Stadejek, T. Aitali,
T. Drew, A. Rebel, S. Yuan, N. Van Long, G. Behrens. Laboratory of Virology, Faculty of Veterinary
Medicine, Ghent University, Salisburylaan, Merelbeke, Belgium.
POSTER ABSTRACTS
Virus-Host Interaction and Pathogenesis
Abstract/Poster No.
6- PRRSV receptor gene CD163 is associated with antibody of PRRSV and SFV.
Q. Tong, X.D. Liu, L.G. Yang, Y. Yu, C. Wang, J.J. Wu, S.H. Zhao and S.J. Zhang Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan, China.
7- Pulmonary dysfunctions in pigs experimentally infected with PRRSV.
J. Wagner*, A. Kneucker, M. Glaser, P. Reinhold. Institute of Molecular Pathogenesis, Friedrich-Loeffler- Institut, Jena, Germany.
8- Establishment of a porcine alveolar macrophage cell line stably expressing porcine CD163 permissive for PRRSV replication.
Y. Lee, E. Nam, and C. Lee. Department of Microbiology, Kyungpook National University, Daegu, South Korea.
9- Modulation of type I interferon production and evasion strategies of PRRSV from the host defense.
D. Yoo*1, O. Kim1, C. Song1, Y. Sun1, Y. Du1, and H.C. Liu2. 1Dept. of Pathobiology, University of Illinois at Urbana-Champaign, Urbana, IL..2Dept. of Animal Science, North Carolina State University, Raleigh, NC.
10- Analysis of 39 porcine type I interferons: expression and activity against PRRSV.
Y. Sang1*, R.R.R. Rowland2, F. Blecha1. Departments of 1Anatomy and Physiology, and 2Diagnostic Medicine and Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS.
11- Using mathematical models to gain insight into host pathogen interactions during PRRSV infection.
A.B. Doeschl-Wilson1*, Lucina Galina-Pantoja2. 1Sustainable Livestock Systems, Scottish Agricultural College, Edinburgh, UK; 2 Pig Improvement Company, Hendersonville, TN, USA. Now at Pfizer Animal Health, US Swine Veterinary Services.
12- PRRS Host Genetics Consortium: Current Progress.
J.K. Lunney1*, J.P. Steibel2 J. Reecy3, M. Rothschild3, M. Kerrigan4, B. Trible4, R.R.R. Rowland.4 1USDA, ARS, BARC, APDL, Beltsville, MD; 2Depts. Animal Science, and Fisheries and Wildlife, Michigan State Univ., East Lansing, MI; 3Dept. Animal Science, Iowa State Univ., Ames, IA; 4Dept Diagnostic Medicine and Pathobiology, Kansas State Univ., Manhattan, KS.
13- Characterization of PCV2 growth in R1BL cells.
Sumathy Puvanendiran and Michael P. Murtaugh. Department of Veterinary and Biomedical Sciences, University of Minnesota, St. Paul, MN.
14- Results of a program of live virus vaccine and avian specific immunoglobulins against PRRSv to stabilize a farm and improve productive parameters.
JD Herrera*1, E. Lucio1, W. González1, J. Chapa1, C. Pérez2, A. Castillo2, JM Doporto.2 1 Investigación Aplicada S.A. de C.V, Tehuacan, Mexico; 2 Independent advisors.
17- An infection model for the study of PRRS at the population level.
R.R. R. Rowland1*, M. Kerrigan1, S. Bujuru1, B. Trible1, J.K. Lunney2, 1Dept Diagnostic Medicine and Pathobiology, Kansas State Univ., Manhattan, KS; 2USDA, ARS, BARC, APDL, Beltsville, MD.
18- Quantitative changes of sialoadhesin and CD163 positive macrophages in the implantation sites and organs of porcine embryos/fetuses during gestation.
Uladzimir U. Karniychuk* and Hans J. Nauwynck. Laboratory of Virology, Department of Virology, Parasitology and Immunology, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.
19- The M/GP5 glycoprotein complex of porcine reproductive and respiratory syndrome virus binds the sialoadhesin receptor in a sialic acid-dependent manner.
Wander Van Breedam1*, Hanne Van Gorp1, Jiquan Q. Zhang2, Paul R. Crocker2, Peter L. Delputte1 and Hans J. Nauwynck1. 1Laboratory of Virology, Department Virology, Parasitology and Immunology, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium. 2Division of Cell Biology and Immunology, Wellcome Trust Biocentre, University of Dundee, Dundee, Scotland, UK.
20- Abstract withdrawn
21- Porcine reproductive and respiratory syndrome virus (PRRSV) nonstructural protein 1 (nsp1) functions as an interferon antagonist.
Z. Chen1, X. Zhou1, Z. Sun1, S. Lawson1, E.A. Nelson1, J. Christopher-Hennings1, Y. Fang1, 2. 1Dept. of Vet. Science, 2Dept. of Biology/Microbiology, SDSU, Brookings, SD.
22- Analysis of mutations in ovarian tumor domain (OTU) of PRRSV nonstructural protein 2.
Z. Sun1, Z. Chen1, X. Zhou1, Y. Fang1, 2. 1Dept. of Vet. Science, 2Dept. of Biology/Microbiology, SDSU, Brookings, SD.
23- Amino acid substitutions in the selected nonstructural and structural proteins of PRRSV determine the viral virulence in pigs.
W. Kim1*, D. Sun1, Y. Cho1, S. Liu1, A.T. Loynachan2, V.C. Cooper1, K.J. Yoon1. 1College of Veterinary Medicine, Iowa State University, Ames, IA. 2Department of Veterinary Science, University of Kentucky, Lexington, KY.
24- Transcriptional Dynamics in PRRSV-infected tissues.
C. W. Beattie*1, S. L. Brockmeier2 and E. F. Retzel3. 1Dept. of Surgical Oncology, University of Illinois- Chicago, Chicago, IL. 2USDA ARS National Animal Disease Center, Ames, IA. 3Genomics and Bioinformatics, National Center for Genome Resources, Santa Fe, NM.
25- Analysis of type-I IFN pathway activation in PAMs with chimeric PRRSV strains. C.Overend1*, R. Maganti1, M.J. Grubman2, A.E. Garmendia.1 1Department of Pathobiology and Veterinary Science, University of Connecticut, Storrs, CT. 2Plum Island Animal Disease Center, USDA-ARS, Greenport, NY.
26- The porcine reproductive and respiratory syndrome virus requires trafficking through CD163 positive early endosomes, but not late endosomes, for productive infection.
Hanne Van Gorp*, Wander Van Breedam, Peter L. Delputte and Hans J. Nauwynck. Laboratory of Virology, Department of Virology, Parasitology and Immunology, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.
27- PRRSV NSP 1ß inhibits host type I IFN response by antagonizing IRF3 activation.
Lalit K. Beura*1, Saumendra N. Sarkar2, Sakthivel Subramaniam1, Byungjoon Kwon1, Clinton Jones1, Asit K. Pattnaik1, Fernando A. Osorio1. 1Nebraska Center for Virology and Dept. of Veterinary Biomedical Sciences, Univ. of Nebraska, Lincoln, NE, 2University of Pittsburgh Cancer Institute and Department of Microbiology and Molecular Genetics, University of Pittsburgh School of Medicine, Pittsburgh, PA.
28- Increased cytotoxicity is observed following in vitro co-infection of cells with porcine reproductive and respiratory syndrome virus and Actinobacillus pleuropneumoniae.
Cynthia Lévesque, Carl A. Gagnon, Mario Jacques. Groupe de recherche sur les maladies infectieuses du porc (GREMIP) and Centre de recherche en infectiologie porcine (CRIP), Faculté de médecine vétérinaire, Université de Montréal, Saint-Hyacinthe, Quebec, Canada.
29- Interaction of PRRSV Nsp1 and the cellular transcription factor PIAS1 (protein inhibitor of STAT1).
C. Song1,4, H. C. Liu2, P. Krell3, and D. Yoo4. 1Dept. of Pathobiology, University of Guelph, Canada. 2Dept. of Animal Science, College of Agriculture and Life Sciences, North Carolina State University. 3Dept. of Molecular and Cellular Biology, University of Guelph, Canada. 4Dept. of Pathobiology, University of Illinois at Urbana-Champaign, Urbana, IL.
30- Identification of immunodominant T-cell epitopes present in matrix protein of the highly pathogenic porcine reproductive and respiratory syndrome virus strain HuN4.
Ya-Xin Wang1, Yan-Jun Zhou1, Guo-Xin Li1, Shan-Rui Zhang1, Yi-Feng Jiang1, Ao-Tian Xu1, Hai Yu1, Meng-Meng Wang1, Li-Ping Yan1, Zong-Yan Cheng1, Zhi-Jun Tian2, Guang-Zhi Tong.1* 1Division of Swine Infectious Diseases, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, China; 2National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China.
31- Porcine reproductive and respiratory syndrome virus controls host immune cells response via Foxp3 expression and AKt pathway.
V. Lazar*, R. Pogranichniy, Animal Disease Diagnostic Laboratory, Department of Comparative Pathobiology, Purdue University, W. Lafayette, IN, USA.
32- Comparison of two cell lines for the propagation of PRRSV.
Melissa Pires-Alves*, Anisha Misra, Federico A. Zuckermann and William Laegreid.
Department of Pathobiology, College of Veterinary Medicine at University of Illinois at Urbana- Champaign, Urbana, IL.
33- Inhibition of type I interferon signaling by Nsp11 of PRRSV.
Y. Sun*, N. Chen, D. Yoo. Department of Pathobiology, College of Veterinary Medicine, University of Illinois at Urbana-Champaign, Urbana, IL.
Molecular Biology: Structural and Functional Relationship
Abstract/Poster No.
34- Large-Scale Parallel Pyrosequencing Technology Reveals PRRSV VR-2332 Nsp2 Deletion Mutants Stable in Swine.
Baoqing Guo, Kay S. Faaberg, Kelly M. Lager, and Marcus E. Kehrli, Jr. National Animal Disease Center- USDA-ARS, Ames, IA.
35- The role of N-glycosylation of minor envelope glycoproteins of PRRSV on infectious virus production and interaction with the receptor CD163.
Phani B. Das*, Phat X. Dinh, Hiep L. X. Vu, Israrul H. Ansari, Byungjoon Kwon, Fernando A. Osorio, and Asit K. Pattnaik. Department of Veterinary and Biomedical Sciences, University of Nebraska-Lincoln.
36- Effects of Nsp2 Deletions on PRRSV Genome and Replication Efficiency.
Raymond K.H. Hui*, Kimberly X. Wang and Frederick C. Leung. School of Biological Sciences, The University of Hong Kong.
37- Cis-Acting Elements in the 3'terminus of the genome of Porcine Reproductive and Respiratory Syndrome Virus are required for viral RNA synthesis.
Changlong Liu, Zhi Sun, Haifeng Yuan, Fei Gao, Jiaqi Lu, Shishan Yuan* Department of Swine Infectious Diseases, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, China.
38- Functional Dissection of The Nucleocapsid Protein of Porcine Arterivirus.
Feifei Tan*, Zuzhang Wei, Chunyan Chen, Rong Zhang, and Shishan Yuan. Department of Swine Infectious Diseases, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences. Shanghai, China.
39- The structural proteins of the Chinese highly pathogenic porcine reproductive and respiratory syndrome virus do not contribute to its virulence.
Jialong Zhang, Lei Zhou, Xin Guo, Xinna Ge, Hanchun Yang*. College of Veterinary Medicine, China Agricultural University, Beijing, P.R. China.
40- Comparative Analysis of Five Pairs of Parental Virulent/ Live Attenuated Vaccine Strains of North-American Type Porcine Reproductive and Respiratory Syndrome Virus: Insight of the Molecular Mutations during the Virulence Attenuation.
Tong-Qing An1, Zhi-Jun Tian1, Yan Xiao1, Yi Zhang1, Ran Li1, Yao Wang1, Jin-Mei Peng1, Yan-Jun Zhou1, Guang-Zhi Tong.1, 2* 1Division of Swine Infectious Diseases, National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China; 2Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, China.
41- Expression of ORF 6 protein of Porcine reproductive and respiratory syndrome virus.
Jitka Janková, Vladimír Celer. University of Veterinary and Pharmaceutical Sciences Brno, Department of Microbiology and Immunology, Palackého, Brno, Czech Republic.
42- Optimization of PCR-based assays for detection of PRRSV in oral fluids.
W Chittick,* W Stensland, J Prickett, E Strait, W-I Kim, K Yoon, JJ Zimmerman. Veterinary Diagnostic Laboratory, College of Veterinary Medicine Iowa State University, Ames, Iowa.
43- Comparison and Analysis of Genome Sequence of Passages PRRSV Strains.
J.Luoa2 , Y.B. Ning1*,Y.B.Liu1, J. Chen3 ,M.Y. Han1, L. Zhang.1 1China Institute of Veterinary Drug Control, Beijing, China; 2 Huazhong Agriculture University, Wuhan, China; 3 Guangdong Minsun Bio- pharmaceutical Co., LTD, Guangzhou, China.
44- Myristoylation of the small envelop (E) protein of PRRS virus is non-essential for virus infectivity but affects its growth.
Y. Du, D. Yoo. Department of Pathobiology, University of Illinois at Urbana-Champaign, Urbana, IL, USA.
45- The GP4 protein of PRRSV is a glycosylphosphatidylinositol (GPI)-anchored protein which contributes to virus infectivity.
Y. Du, D. Yoo. Department of Pathobiology, University of Illinois at Urbana-Champaign, Urbana, IL, USA.
46- Gene rearrangements for structural proteins of PRRSV and a novel approach to immunogenic alteration.
C. Song1,3, P. Krell2, Y. Du3, and D. Yoo3. 1Depts. of Pathobiology, and 2Molecular and Cellular Biology, University of Guelph, Canada. 3Dept. of Pathobiology, University of Illinois at Urbana-Champaign, Urbana, IL.
47- Generation of an infectious clone of HuN4-F112, an attenuated live vaccine strain of highly pathogenic porcine reproductive and respiratory syndrome virus.
Shan-Rui Zhang, Yan-Jun Zhou, Zhi-Jun Tian, Hai Yu, Guo-Xin Li, Li-Ping Yan, Yi-Feng Jiang, Ya-Xin Wang, Meng-Meng Wang, Ao-Tian Xu, Guang-Zhi Tong* Division of Swine Infectious Diseases, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, China.
48- Genetic characterization of a novel, highly virulent PRRSV isolate from North America.
J. C. Schwartz1*, R. G. Jones2, J. E. Abrahante1, M. P. Murtaugh1. 1Department of Veterinary and Biomedical Sciences, University of Minnesota, St. Paul, MN. 2Livestock Veterinary Services, Kinston, NC.
49- Development of a DAN-launched reverse genetics system for Korean type 1 porcine reproductive and respiratory syndrome virus.
E. Nam*, M. Sagong, C. Lee. Department of Microbiology, Kyungpook National University, Daegu, South Korea.
50- Viral N-acetylglucosamine Facilitates Porcine Reproductive and Respiratory Syndrome Virus Infection of Permissive Cells.
J. Li*, M. P. Murtaugh. Dept. of Veterinary and Biomedical Sciences, University of Minnesota, St. Paul, MN.
Epidemiology, Heterogeneity and Evolution
Abstract/Poster No.
51- Spontaneous deletion of nsp2 region associated with cell-adaptation of porcine reproductive and respiratory syndrome virus.
Shishan Yuan1*, Xiaomin Wang1,2, Zuzhang Wei1, Yanhua Li1, Xiangjian Li1, Huochun Yao2 .1 Department of Swine Infectious Diseases, Shanghai Veterinary Research Institute, The Chinese Academy of Agricultural Sciences, Shanghai, PR China; 2 College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu Province, PR China.
52- PRRSV receptor level and susceptibility to PRRSV infection increased on cultured peripheral blood monocytes.
Liang-hai Wang* and Wen-hai Feng. State key laboratory for Agrobiotechnology, and Department of Microbiology and Immunology, College of Biological Science, China Agricultural University, Beijing, China.
53- The current antigenic heterogeneity between PRRSV strains can influence the reliability of serological surveys.
Uladzimir U. Karniychuk1*, Marc Geldhof1, Merijn Vanhee1, Jan Van Doorsselaere2, Hans J. Nauwynck1. 1Laboratory of Virology, Department of Virology, Parasitology and Immunology, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium. 2Department of Health Care and Biotechnology, KATHO Catholic University College of South-West Flanders, Roeselare, Belgium.
54- An Outbreak of the Highly Pathogenic American PRRS Virus of the Chinese Strain in the Philippines.
Leonilo R. Resontoc, DVM, MPA, Bureau of Animal Industry, Department of Agriculture, Diliman, Quezon City, Philippines.
55- A Phylogeny-based Demographical and Geographical Dissection of North American Type Porcine Reproductive and Respiratory Syndrome Viruses.
1Mang Shi, 1Tsan-Yuk Lam, 1Chung-Chau Hon, 2Michael P. Murtaugh, 2Peter Davies, 3 Raymond Kin-Hei Hui, 1Lina Tik-Wim Wong, 1Chi-Wai Yip, 1Jin-wai Jiang, and 1*Frederick Chi-Ching Leung 1School of
Biological Sciences, The University of Hong Kong, China; Departments of 2Veterinary and Biomedical Sciences, and 3Veterinary Population Medicine, University of Minnesota, Minnesota, USA.
56- Characterization of the GP4-specific antibody response upon infection with PRRSV.
M. Vanhee, S. Costers* and H.J. Nauwynck Laboratory of Virology, Department of Virology, Parasitology and Immunology, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.
57- Evidence of long distance airborne transport of porcine reproductive and respiratory syndrome virus and Mycoplasma hyopneumoniae.
S Dee, S Otake, S Oliveira and J Deen, University of Minnesota Swine Disease Eradication Center,
St Paul, MN.
58- Use of a production region model to evaluate aerosol transmission and biosecurity of PRRSV and Mycoplasma hyopneumoniae: Results from a 3-year study.
S Dee, S Otake and J Deen, University of Minnesota Swine Disease Eradication Center, St Paul, MN.
59- A pilot study to evaluate the efficacy of air filtration in large sow herds located in swine- dense regions.
Scott Dee1, Gordon Spronk2 and Peter Davies1, 1Swine Disease Eradication Center, University of Minnesota, 2Pipestone Veterinary Clinic, Pipestone, MN.
60- UV254 Inactivation of Selected Viral Pathogens.
TD Cutler,1 SJ Hoff,3 C Wang,1,5 KJ Warren,4 F Zhou,1 Q Qin,2 C Miller2, JF Ridpath,6 K-J Yoon,1 J Zimmerman1 1Department of Veterinary Diagnostic and Production Animal Medicine, 2Department of Veterinary Microbiology and Preventive Medicine, 3Department of Agricultural and Biosystems Engineering, 4Department of Immunobiology 5Department of Statistics, Iowa State University, Ames, Iowa. 6USDA:ARS:NADC, Ames, Iowa.
61- Infectious dose (ID50) of PRRSV Isolate MN-184 for Young Pigs via Aerosol Exposure.
TD Cutler,1 A Kittawornrat,1 SJ Hoff,2 C Wang,1,3 JJ Zimmerman1 1Department of Veterinary Diagnostic and Production Animal Medicine, 2Department of Agricultural and Biosystems Engineering, 3Department of Statistics, Iowa State University, Ames, Iowa.
62- Torque teno virus (TTV) in commercial wean-to-finish populations concurrently infected with PRRSV.
J Prickett,1* R Pogranichniy,2 R Main,1 A Clark2, J Zimmerman1 1College of Veterinary Medicine, Iowa State University, Ames, IA. 2Animal Disease Diagnostic Laboratory, Department of Comparative Pathobiology, Purdue University, West Lafayette, IN.
63- Abstract withdrawn
64- PRRS Regional Eradication in Minnesota.
Cesar Corzo*, Bob Morrison, Department of Veterinary Population Medicine, College of Veterinary Medicine University of Minnesota, Saint Paul, MN.
65- Potential origin of the newly emerged highly pathogenic porcine reproductive and respiratory syndrome virus in China.
Tong-Qing An1, Zhi-Jun Tian1, Yan Xiao1, Ran Li1, Jin-Mei Peng1, Tian-Chao Wei1, Yi-Feng Jiang1, Guang-Zhi Tong1,2,* 1Division of Swine Infectious Diseases, National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China; 2 Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, China.
66- Prevalence and correlation between PRRS and porcine TT viruses in the Czech Republic.
Veronika Jarošová, Jitka Janková, Vladimír Celer. University of Veterinary and Pharmaceutical Sciences Brno, Department of Microbiology and Immunology, Palackého, Brno, Czech Republic.
67- Collection of oral fluid diagnostic samples from individual boars.
A Kittawornrat,1* M Engle,2 J Johnson,1 D Whitney,1 C Olsen,1 J Prickett,1 T Schwartz,1 K Schwartz,1 J Zimmerman1 1College of Veterinary Medicine, Iowa State University, Ames, IA. 2PIC North America, Hendersonville, TN.
68- PRRSV whole genome sequencing via next-generation sequencing.
Juan E. Abrahante, John C. Schwartz and Michael P. Murtaugh. Department of Veterinary and Biomedical Sciences, University of Minnesota, St. Paul, MN.
69- High fever disease: genetic variation and pathogenicity of porcine reproductive and respiratory syndrome virus emerged in China.
J. Wu1*, J. Li1, F. Tian2, S. Ren1, J. Chen2, Z. Lan2, X. Zhang1, D. Yoo3, Z. Niu4, J. Wang1. 1Shandong Key Laboratory of Animal Diseases Control and Breeding, Shandong Academy of Agricultural Sciences, Jinan, Shandong, China. 2Shandong Center for Animal Disease Control and Prevention, Jinan, Shandong, China. 3Dept. of Pathobiology, University of Illinois at Urbana-Champagne, IL, USA.
70- Characterization and pathogenicity of one PRRSV strain with 1-amino-acid deletion at position 481 of Nsp2.
Yanhua Li, Shuxian Chen, Lei Zhou, Jialong Zhang, Xin Guo, Xinna Ge, Hanchun Yang*. College of Veterinary Medicine, China Agricultural University, Beijing, P.R.China.
72- A Web-based Database and Phylogenetic Tools to Study Molecular Epidemiology and Evolution of Porcine Reproductive and Respiratory Syndrome Virus (PRRSV).
Jun Li, Charles Lai-Yin Wong, Tommy Tsan-Yuk Lam, Mang Shi, Frederick Chi-Ching Leung*. School of Biological Sciences, The University of Hong Kong, Hong Kong SAR, China.
73- PriProET based melting point analyses on PRRSV positive field samples.
Gyula Balka1,*, Ádám Dán2, Ákos Hornyák3, Andrea Ladinig4, Miklós Rusvai.1 1Department of Pathology and Forensic Veterinary Medicine, Faculty of Veterinary Science, Szent István University, Budapest, Hungary; 2 Central Agricultural Office, Veterinary Diagnostic Directorate, Budapest, Hungary; 3 Department of Microbiology and Infectious Diseases, Faculty of Veterinary Science, Szent István University, Budapest, Hungary; 4 Clinic for Swine, Clinical Department for Farm Animals and Herd Health, University of Veterinary Medicine Vienna, Austria.
74- Molecular epidemiology of PRRS virus infections in Britain.
J.P. Frossard*, S. Williamson, D. Westcott, B. Naidu, T. Drew. Veterinary Laboratories Agency (Weybridge), New Haw, Addlestone, Surrey, U.K.
75- The first case of porcine reproductive and respiratory syndrome in Bhutan.
T. Stadejek1*, V. Raika2, K. Chabros1, K. Rinzin2, S. Parchariyanon3. 1Department of Swine Diseases, OIE Reference Laboratory for PRRS, National Veterinary Research Institute, Pulawy, Poland. 2National Centre for Animal Health, Thimpu, Bhutan. 3National Institute of Animal Health, Bangkok, Thailand.
Vaccine and Immunity
Abstract/Poster No.